Optimisation of extraction procedure and .Deze pagina vertalen
Briefly, three rows of kernels were snap frozen by liquid nitrogen and cryo-milled using a ball mill (MM400 Retsch Mixer Mill, Haan, Germany) operated at 30 Hz for 60 s. The powdered sample (about 0.5 g) was transferred to a 15-mL Falcon® tube, 120 µL of IS and 3.88 mL of cold extraction solution B .
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